02
Mar

Drug treatment of breast cancer tumoroids in an automated microfluidic system

A recent paper from Cromwell et al. in Burow lab in Tulane university modeling multifunctional profile of breast cancer tumoroids to  investigate the effect of relative anti-cancer drugs on tumoroid phenotype and cell metabolism function. Authors initially cultured cells isolated from patients in conventional cell culture dishes and later in a low attachment U-shaped wells. They used magnetic nanoparticles to coat tumors into “flowchip wells”. The wells are connected to other wells with different reagents through microchannels, flow of reagents is controlled by pneumatic pumps.

They concluded that exposed tumors by drugs had different phenotypes like lower average intensities of stainings and cellular areas showing less cell viability. Moreover, Lactate secretion increased during drug treatment which showed the cells notably shifted their metabolic pathway and tumors underwent anaerobic glycolysis. The result of energy metabolism was measured by ATP based luminance assay.

Strong points:

+ Highly heterogenous phenotype of breast cancer primary cell.
+ automated sampling assay
+ Different drug treatment with different mechanisms of action.

Negative points:

– Lack of physiological tumor microenvironment
– Still labor-intensive preparation of tumoroids

Link to paper: https://www.sciencedirect.com/science/article/pii/S2472555222000119

This article is reproduced under https://creativecommons.org/licenses/by/4.0/