Drug treatment of breast cancer tumoroids in an automated microfluidic system
A recent paper from Cromwell et al. in Burow lab in Tulane university modeling multifunctional profile of breast cancer tumoroids to investigate the effect of relative anti-cancer drugs on tumoroid phenotype and cell metabolism function. Authors initially cultured cells isolated from patients in conventional cell culture dishes and later in a low attachment U-shaped wells. They used magnetic nanoparticles to coat tumors into “flowchip wells”. The wells are connected to other wells with different reagents through microchannels, flow of reagents is controlled by pneumatic pumps.
They concluded that exposed tumors by drugs had different phenotypes like lower average intensities of stainings and cellular areas showing less cell viability. Moreover, Lactate secretion increased during drug treatment which showed the cells notably shifted their metabolic pathway and tumors underwent anaerobic glycolysis. The result of energy metabolism was measured by ATP based luminance assay.
+ Highly heterogenous phenotype of breast cancer primary cell.
+ automated sampling assay
+ Different drug treatment with different mechanisms of action.
– Lack of physiological tumor microenvironment
– Still labor-intensive preparation of tumoroids
This article is reproduced under https://creativecommons.org/licenses/by/4.0/