23
Feb

Oxygen monitoring in a liver on a chip to study effect of drugs and nutrients

A nice collaborative paper from Stelzle, Gebhardt and Mayr labs in U. of Tübingen, Leipzig University and TU Graz monitoring oxygen and liver tissue metabolism in a well-plate format organ on a chip system. They used oxygen patches and optical fibers to monitor oxygen levels in the medium around the liver tissue made from primary human hepatocytes. They changed the medium every 14 h by adjusting the medium height levels in the reservoirs.

They refreshed the medium every 14 h by adjusting the medium height levels in the reservoirs. The authors monitored the oxygen in the medium inside the liver on a chip and studied the effect of nutrients, fructose among others, on hepatocyte energy metabolism. They found out that fructose can have adverse effect on cellular respiration. Moreover they concluded that flow rate can be considered as an important factor to balance oxygen consumption.

Strong points:
+ Real-time monitoring of oxygen consumption.
+ Integrated electrodes
+ Biocompatible low adsorption materials

Nothing is perfect! The system can also improve:
– Manual perfusion
– Variable flow rate
– One optical fiber for each chip, so 24 wires connected to the plate

Link to the paper: https://www.excli.de/index.php/excli/article/view/4351

This article is reproduced under https://creativecommons.org/licenses/by/4.0/