Liver pancreas crosstalk in organ on a chip to study type 2 Diabetes

A beautiful paper from Jianhua Qin’s lab from the Chinese Academy of Sciences modelling Liver-pancreatic Islet Axis. They used a multi-organoid organ on a chip system to study glucose regulation in physiological and pathological conditions. After culturing the hiPSCs in “micro-wells” and creating embryonic bodies, the authors differentiated them to two lineages of liver and pancreatic islets. Then, they co-cultured these self-assembled differentiated organoids in two different compartments in an organ on a chip and compared liver or islet mono-culture with the co-culture. They performed glucose tolerance test (GTT) to assess glucose utilization. They found out that the co-culture of liver and pancreatic Islets was able regulate glucose consumption and albumin secretion more efficiently compared to the mono-cultures. Moreover, they showed that in hyperglycemic conditions, Metformin treatment restored the respiratory capacity, ATP function and glucose transport functions in both liver and islet organoids. This novel study provides us a unique platform for Type 2 Diabetes mellitus (T2DM) research.

Strong points:
+ Comprehensive differences between co-culture and mono-cultures
+ Using hiPSCs-derived organoids
+ Compartmentalized chip

Nothing is perfect! The system can also improve:
– The same old story, they used PDMS but performed no investigation of the Metformin absorption into PDMS
– Lack of control on the number of organoids cultured in the chip (No data shown)

If you have any questions, feel free to contact us!

Link to the paper: https://onlinelibrary.wiley.com/doi/full/10.1002/advs.202103495

Organ on a chip, Multi-organ microphysiological systems, Microfluidics multi-organ on a chip, organ-organ interaction, iPSC, pancreas, Liver, pancreatic islet, type 2 diabetes

This paper is reproduced under https://creativecommons.org/licenses/by/4.0/. The image of the chip was edited for better clarity, data in the table and text were compiled and interpreted by AZAR Innovations.