3D pancreatic tumor spheroids co-cultured with stromal cells in an organ on a chip

A recent paper from Jang et al. from Kuh’s lab at the Catholic University of Korea co-culturing pancreatic cancer cells with fibroblasts and macrophages in 3D in an organ on a chip. The paper evaluates the culture of the cancer cells with and without the stromal cells and studies the anti-invasive activity and cell-type dependent cytotoxicity of chemotherapeutic drugs.

Authors found that epithelial-mesenchymal transition (EMT) related protein in pancreatic tumor spheroids is increased when co-cultured with stromal cell. Furthermore, after treatment with 4 drugs, Gemcitabine ,5-fluorouracil, Oxaliplatin, and Paclitaxel, the authors concluded that cell viability and invasiveness depended on the drug and its dosage.

Strong points:
+ 3D co-culture of tumor spheroids with stromal cell

+ Study of different drugs with different concentration
+ Analyzing 3D spatial and temporal heterogeneity of subpopulation
+ Compartmentalized chip

Nothing is perfect! The system can also improve:
– The same old story, PDMS but no investigation on the real concentration that the organoids are exposed to and what fraction of the drug is absorbed into PDMS
-They used cancer and monocytic cell lines
-No automatic perfusion, cell culture was refreshed by hand

Would you like to know more or do you want to develop your own 3D tumor model on a chip, contact us!

Link to the paper: https://www.mdpi.com/2072-6694/13/23/5955

This article is reproduced under https://creativecommons.org/licenses/by/4.0/